Amino acid-producing bacteria and a process for preparing...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12N 15/52 (2006.01) C12P 13/04 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2478266

The invention relates to amino acid-producing bacteria from the family Enterobacteriaceae, in particular from the species Escherichia coli, which contain a stop codon chosen from the group amber, ochre and opal in the nucleotide sequence for the coding region of the rpoS gene and a suppressor for a stop codon chosen from the group amber suppressor, ochre suppressor and opal suppressor. This invention also relates to a process for preparing amino acids, in particular L-threonine, using these bacteria.

L'invention concerne une bactérie productrice d'aminoacides issue de la famille des entérobactériacées, et notamment de l'espèce Escherichia coli, et qui contient un codon d'arrêt choisi dans le groupe constitué par l'ambre, l'ocre et l'opale dans la séquence nucléotidique pour la région codante du gène rpoS, ainsi qu'un suppresseur pour un codon d'arrêt choisi dans le groupe constitué par le suppresseur d'ambre, le suppresseur d'ocre et le suppresseur d'opale. L'invention concerne également un procédé de préparation d'aminoacides, et notamment de L-thréonine, au moyen de cette bactérie.

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