Hiv p24 quantitation panel

G - Physics – 01 – N

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G01N 33/569 (2006.01) C07K 14/16 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2018098

ABSTRACT The present invention is directed to improved immunoassay methods, and compositions and kits for carrying out the methods, for quantitating HIV p24 antigen in a biological sample. The method involves contacting a sample with an antibody specific for HIV p24 antigen, whereby a detectable antigen-antibody complex is formed, and quantitating the amount of the complex formed by employing a quantitation panel as a standard with which the amount of the complex formed can be compared. The method involves utilizing purified HIV p24 antigen in the quantitation panel. Preferably, the quantitation panel comprises lyophilized standards containing immunoaffinity-purified viral p24 antigen. The composition includes purified viral HIV p24 and a buffer. The kit includes a panel of purified viral HIV p24 compositions. 14

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