Method and apparatus for liquid chromatography automated...

G - Physics – 01 – N

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G01N 1/00 (2006.01) B01L 3/02 (2006.01)

Patent

CA 2549543

A method for automated loading of liquid sample to a liquid chromatography testing device is provided including installing a disposable tip on a probe, moving the probe with an automated liquid handler to a loading position proximate to a sample, and drawing a sample into the disposable tip. The probe is then moved to an injection position proximate an injection port, and the sample is injected from the disposable tip into the injection port. Also provided are the novel injection ports. After injection of the sample, the disposable tip is then removed from the probe. These steps may be repeated a plurality of times to sequentially load a plurality of samples. An exemplary method of the invention is directed to use with a liquid chromatography apparatus. An apparatus for assisting in the removal of the disposable tip is also provided.

L'invention porte sur un procédé de chargement automatisé d'échantillon liquide dans un dispositif de test par chromatographie liquide, selon lequel on installe une pointe jetable sur une sonde, on place la sonde dans une position de chargement proche d'un échantillon à l'aide d'un manipulateur de liquide automatisé, et on attire l'échantillon jusque dans la pointe jetable. On place alors la sonde dans une position d'injection proche d'un orifice d'injection, et l'échantillon est injecté de la pointe jetable dans l'orifice d'injection. L'invention se rapporte également à de nouveaux orifices d'injection. Après injection de l'échantillon, on enlève la pointe jetable de la sonde. On peut répéter ces étapes plusieurs fois afin de charger séquentiellement une pluralité d'échantillons. Un procédé exemplaire de l'invention fait appel à un appareil de chromatographie liquide. L'invention concerne également un appareil qui facilite le retrait de la pointe jetable.

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