Method of activating denatured protein

Details Method of activating denatured protein Method of activating denatured protein

C - Chemistry, Metallurgy

07

K

C07K 1/113 (2006.01) C07K 14/48 (2006.01) C07K 14/745 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2257122

Proposed is a method of preparing mixed disulphides from a protein and a disulphide component. The method is characterized in that the protein, in an inactive, sparingly soluble form, is incubated with a solution of a denaturing agent at a concentration sufficient for denaturation and in the presence of a disulphide component, dissolved and converted into a derivative. The method is suitable for the high-yield preparation of renaturated recombinant proteins from prokaryotes.

L'invention concerne un procédé de préparation de disulfures mélangés, à partir d'une protéine et d'un composant disulfure, ledit procédé étant caractérisé en ce que la protéine sous une forme inactive difficilement soluble, est incubée avec une solution d'un agent dénaturant dans une concentration suffisante pour assurer la dénaturation et en présence d'un composant disulfure, puis dissoute et transformée en un dérivé. Ce procédé peut s'utiliser pour préparer, avec un haut rendement, des protéines recombinées renaturées à partir de procaryotes.

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