Method and kit for monitoring mammalian reproductive cycles

G - Physics – 01 – N

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Details

G01N 33/50 (2006.01) A61B 10/00 (2006.01) G01N 27/26 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2198187

A method for monitoring mammalian reproductive cycles by monitoring variations in the quantity of one or more low molecular weight volatile compounds having a molecular weight of less than 50 grams per mole present in a body constituent sample is disclosed. Samples of a body constituent selected from the group consisting essentially of humoral fluid, breath and body cavity air are collected from a female mammal a multiple number of times during the reproductive cycle. The quantity of a low molecular weight volatile coumpond in each sample is measured. In the preferred embodiment, the low molecular weight volatile compound, acetaldehyde, will be measured and monitored. Variations in the quantity of the low molecular weight volatile compound appearing in each sample is monitored to determine the phase of the mammal's reproductive cycle and to predict the occurrence of ovulation.

On décrit un procédé pour suivre le cycle ovarien chez des mammifères en mesurant les variations de la quantité d'un ou de plusieurs composés volatils à poids moléculaire bas et en l'occurrence, inférieur à 50 g par mole, présents dans un échantillon provenant du mammifère. Comme échantillon, on peut utiliser les fluides extracellulaires, l'air expiré et l'air de cavités du corps, que l'on recueille sur la femelle plusieurs fois durant le cycle ovarien. On mesure la quantité d'un composé volatil à poids moléculaire bas dans chaque échantillon. Dans la forme d'exécution préférée, le composé volatil à poids moléculaire bas mesuré est l'acétaldéhyde. On enregistre les variations de la quantité du composé à poids moléculaire bas dans chaque échantillon, pour déterminer à quel stade du cycle ovarien se trouve le mammifère, et en particulier pour prédire le moment de l'ovulation.

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