A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 1/02 (2006.01) A01N 3/00 (2006.01) A23B 4/20 (2006.01) A23L 3/3526 (2006.01) A61K 38/18 (2006.01) C12N 5/02 (2006.01)
Patent
CA 2076380
2076380 9212722 PCTABS00014 The present invention relates to aqueous compositions of substances, such as organic molecules, which are useful to protect and preserve viable plant or animal cell membrane and tissue exposed to hypothermal and hyperthermal temperatures or nonphysiological chemical conditions, and to modify the freezing process of liquids in biological plant or animal cells or tissue. More specifically, the present invention relates to the use of antifreeze polypeptide or antifreeze glycopeptide which is derived, for example, from the fluid or serum of Artic and Antarctic fish. Preferred antifreeze compounds are related to those polypeptides having multiple alanine-alanine-threonine- or alanine-alanine-alanine- segments. In some embodiments, a pendant sugar group is covalently attached to each threonine moiety. An aqueous solution of the peptide or glycopeptide is contacted with cells ova, sperm, oocytes, embryos, tissue, an organ, or a whole living plant or animal. The cells, tissue, organ or plant or animal is then carefully cooled and/or frozen at 0 ·C or below (in some cases to -196 ·C or to 4 K) and held at the low freezing (or vitrification) temperatures. The ice forms, if at all primarily along the c-axis with the result that cell membranes are not disrupted and the cells are not dehydrated. The cells, tissue, whole plant or organ or animal are carefully thawed, and all are found to be viable. The preserved organs are particularly useful transplant organs for a human being.
Devries Arthur L.
Rubinsky Boris
Devries Arthur L.
Kirby Eades Gale Baker
Rubinsky Boris
The Regents Of The University Of California
LandOfFree
Compositions to improve survival of biological materials does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions to improve survival of biological materials, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions to improve survival of biological materials will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1999987