Alphavirus expression vector

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 31/70 (2006.01) C12N 7/01 (2006.01)

Patent

CA 2188421

The present invention is directed to alphavirus expression vectors comprising at least part of an alphavirus genome and hetetologous RNA inserted downstream of an alphavirus base sequence having translation enhancing activity. Such vectors can be used to achieve enhanced levels of expression of DNA or cDNA coding for a desired product and being complementary to said heterologous RNA after introduction of said vector in eukaryotic cells in cell culture or in a living body. The expression product may have therapeutical or prophylactic activity.

La présente invention concerne des vecteurs d'expression de l'alphavirus comportant au moins une partie d'un génome d'alphavirus et un ARN hétérologue inséré en aval d'une séquence de base d'alphavirus présentant une activité facilitant la traduction. De tels vecteurs s'utilisent pour obtenir des niveaux améliorés d'expression d'ADN ou d'ADNc codant pour un produit désiré et étant complémentaires dudit ARN hétérologue après introduction dudit vecteur dans des cellules eucaryotes dans une culture cellulaire ou dans un organisme vivant. Le produit d'expression peut présenter une activité thérapeutique ou prophylactique.

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