Dihydroxy-acid dehydratase gene and use thereof

Details Dihydroxy-acid dehydratase gene and use thereof Dihydroxy-acid dehydratase gene and use thereof

C - Chemistry, Metallurgy

07

K

C07K 14/435 (2006.01)

Patent

CA 2617734

The present invention relates to an dihydroxy-acid dehydratase gene and use thereof, in particular, a brewery yeast for producing alcoholic beverages having superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast whose level of producing total vicinal diketones, especially level of producing diacetyl, that are responsible off-flavor of the product, is reduced by amplifying the expression level of ILV3 gene encoding brewery yeast dihydroxy-acid dehydratase ILV3p, particularly non-ScILV3 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.

La présente invention concerne un gène de la dihydroxyacide déshydratase et l'utilisation de celui-ci, en particulier une levure de brasserie servant à produire des boissons alcoolisées ayant un arôme supérieur, des boissons alcoolisées produites avec ladite levure et un procédé servant à produire lesdites boissons. Plus particulièrement, la présente invention concerne une levure dont le niveau de production de dicétones vicinales totales, en particulier le niveau de production de diacétyle, qui sont responsables d'une flaveur atypique du produit, est réduit en amplifiant le niveau d'expression du gène ILV3 codant pour la dihydroxyacide déshydratase de levure de brasserie ILV3p, en particulier du gène ILV3 non-Sc spécifique à une levure de brasserie de fermentation basse ; et un procédé servant à produire des boissons alcoolisées avec ladite levure.

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