Cyanobacterial nucleic acid fragments encoding proteins...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01) C07K 14/195 (2006.01) C12N 15/53 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2413793

This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable marking. In particular, it provides for cyanobacteria, for example, Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4'-fluoro-6- [(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.

L'invention concerne des cyanobactéries comme autre source de gènes ahas et pds, pour des transformations végétales et des marquages sélectifs. L'invention concerne notamment des cyanobactéries, par exemple, Synechocystis, comme source de gènes codant pour des protéines insensibles aux herbicides, et d'éléments de gènes pour la régulation d'expression dans des plastides. Des fragments d'acides nucléiques, aussi bien la grande sous-unité d'acétolactate synthase (ahas) et la petite sous-unité ahas, fournissent une résistance aux herbicides. L'invention concerne également un nouveau gène Synechocystis mutant de phytoène désaturase (PDS) conférant une résistance à 4'-fluoro-6-[(alpha, alpha, alpha,-trifluoro-m-tolyl)oxy]-picolinamide, un herbicide de blanchiment. L'invention apporte des améliorations à un procédé faisant appel aux cyanobactéries pour le criblage de composés, et intégrant un nouveau protocole à haut débit qui représente un moyen rapide et financièrement avantageux d'identification de gènes de site cible.

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