G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 21/64 (2006.01) B01L 3/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2440183
The invention relates to a method for determining an analyte in a sample by means of fluorescence correlation spectroscopy. The distance between the measuring volumes in the sample and the optical stimulation/detection direction is >= 1 mm and the sample liquid is thermally isolated from the optical device. The inventive method is especially suitable for measuring temperature-changeable processes, e.g. determining nucleic acid hybridisation melting curves, or for carrying out nucleic acid amplification reactions. The invention also relates to a device for carrying out the inventive method.
L'invention concerne un procédé de détermination d'un analyte dans un échantillon par spectroscopie de corrélation par fluorescence, dans lequel la distance entre le volume de mesure dans l'échantillon et la direction d'excitation/détection optique est >= 1 mm, et le liquide échantillon est isolé thermiquement du dispositif optique. Le procédé convient notamment pour la mesure de processus variant en fonction de la température, par exemple, la détermination des courbes de fusion hybridisation d'acide nucléique, ou pour la mise en oeuvre de réactions d'amplification d'acide nucléique. L'invention concerne en outre un dispositif permettant la mise en oeuvre de ce procédé.
Gnothis Holding Sa
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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