Hybrid microfluidic spr and molecular imaging device

G - Physics – 01 – N

Patent

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G01N 21/94 (2006.01) B81B 1/00 (2006.01) G01N 1/40 (2006.01) G01N 15/00 (2006.01) G01N 33/48 (2006.01) G01N 35/00 (2006.01) G01N 21/15 (2006.01) G01N 21/41 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2704216

A hybrid microfluidic biochip designed to perform multiplexed detection of singled- celled pathogens using a combination of SPR and epi-fluorescence imaging. The device comprises an array of gold spots, each functionalized with a capture biomolecule targeting a specific pathogen. This biosensor array is enclosed by a polydimethylsiloxane (PDMS) microfluidic flow chamber that delivers a magnetically concentrated sample to be tested. The sample is imaged by surface plasmon resonance on the bottom of the biochip, and epi- fluorescence on the top.

La présente invention concerne une biopuce microfluidique conçue pour effectuer une détection multiplexée de pathogènes monocellulaires en utilisant une combinaison de SPR et d'imagerie par épi-fluorescence. Le dispositif comprend une matrice de points d'or, chacun étant fonctionnalisé avec une biomolécule de capture ciblant un pathogène spécifique. Cette matrice de biocapteurs est incorporée dans une chambre de flux microfluidique en polydiméthylsiloxane (PDMS) qui délivre un échantillon à tester concentré magnétiquement. L'échantillon est imagé par résonance du plasmon de surface (SPR) au fond de la biopuce, et par épi-fluorescence au sommet.

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