Use of caspase enzymes for maturation of engineered...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/54 (2006.01) C07K 14/56 (2006.01) C12N 9/64 (2006.01) C12N 15/70 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2511855

The invention relates to methods of producing and isolating recombinant proteins using fusion protein constructs comprising specific recognition sites for a maturating protease. The invention specifically relates to the use of caspase proteases for the maturation of engineered recombinant fusion proteins or polypeptides. These molecules are engineered using recombinant DNA technology to comprise a specific target sequence for a caspase between a first fusion part and the polypeptide of interest. After processing, the desired mature format of the protein or polypeptide is obtained.

Cette invention a trait à des procédés permettant de produire et d'isoler des protéines de recombinaison à l'aide de produits de synthèse de protéines hybrides comportant des sites spécifiques de reconnaissance relatifs à une protéase de maturation. Elle porte plus précisément sur des protéases de caspases aux fins de la maturation de protéines hybrides de recombinaison ou de polypeptides. Ces molécules sont produites par génie génétique utilisant une technique d'ADN de recombinaison afin de comporter une séquence cible particulière relative à des caspases entre une première partie hybride et le polypeptide à étudier. On obtient, après traitement, la forme mature de la protéine ou du polypeptide souhaitées.

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