Use of herpes simplex virus orf p protein in repression of...

A - Human Necessities – 61 – K

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A61K 38/16 (2006.01) A61K 31/70 (2006.01) A61K 39/42 (2006.01) A61K 48/00 (2006.01) C07K 1/22 (2006.01) C07K 14/035 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2265088

The present invention is directed to methods and compositions relating to the treatment of herpes simplex virus infections and the screening of compounds for activity that inhibit or promote viral latency. The previously identified ORF P gene product now has been shown to interact with certain eukaryotic splicing factors and, in a cell infected with a herpesvirus containing a derepressed ORF P gene, ORF P can limit the splicing of at least two viral products. Given this function, it now is possible to screen for inhibitors and inducers of ORF P and, further, provide methods for maintaining and preventing viral latency.

Cette invention se rapporte à des procédés et à des compositions concernant le traitement des infections par le virus de l'herpès simplex et concernant le criblage de composés pour déterminer lesquels ont une activité inhibant ou stimulant la latence virale. On a pu établir que le produit génique ORF P préalablement identifié interagit avec certains facteurs d'épissage eukaryotique et, dans une cellule infectée par un virus de l'herpès contenant un gène ORF P déréprimé, la protéine ORF P peut limiter l'épissage d'au moins deux produits viraux. Cette fonction ayant été établie, il est actuellement possible de cribler les agents inhibiteurs et inducteurs de la protéine ORF P et d'obtenir en outre des procédés pour entretenir ou prévenir la latence virale.

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