Dna sequences for the detection of and differentation...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2566336

Oligonucleotide sequences and methods for specifically detecting and differentiating amongst pathogenic E.coli in a complex sample. The complex sample can be a food sample, water sample, or selectively enriched food matrix. The methods of detection may utilize PCR amplification with, or without, an internal positive control, and appropriate primer pairs. Reagents for performing the methods can be supplied as a kit and/or in tablet form.

L'invention concerne des séquences d'oligonucléotides et des méthodes de détection et de différenciation spécifiques entre desE.coli pathogènes dans un échantillon complexe. L'échantillon complexe peut être un échantillon alimentaire, un échantillon d'eau ou une matrice d'aliments enrichie sélectivement. Les méthodes de détection peuvent utiliser une amplification ACP avec ou sans témoin positif interne ainsi que des paires d'amorces appropriées. Des réactifs de mise en application des méthodes peuvent être fournis sous la forme d'un kit et/ou de pastilles.

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