Method for labeling biopolymers using isotopes

A - Human Necessities – 61 – K

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A61K 51/12 (2006.01) A61K 49/00 (2006.01) C12P 19/34 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2337869

The invention relates to a novel method for the in-vivo labeling of biopolymers such as proteins, nucleic acids, lipids, carbohydrates and biodegradable plastic using isotopes, especially stabile isotopes. The invention also relates to the use of chemolithotrophic bacteria for the in- vivo isotopic labeling of biopolymers. In particular, the invention relates to the use of CO2-fixing bacteria, such as Ralstonia eutropha and related methanogenic bacteria for in-vivo isotopic labeling, especially for labeling using the stable isotope 13C alone and in combination with other isotopes. In addition, the invention relates to the use of the isotropically labeled biomolecules in therapeutic and diagnostic applications, especially in spectroscopic methods and generally as tracers.

L'invention concerne une nouvelle technique qui permet de marquer in vivo des biopolymères, tels que protéines, acides nucléiques, lipides, glucides et plastiques biodégradables, par des isotopes, notamment des isotopes stables, et l'utilisation de bactéries autotrophes pour marquer des biopolymères in vivo. Elle concerne notamment l'utilisation de bactéries fixant le CO¿2?, tels que Ralstonia eutropha et les bactéries méthanogènes apparentées, pour le marquage isotopique in vivo, notamment pour le marquage par l'isotope stable ?13¿C, seul ou associé à d'autres isotopes, ainsi que l'utilisation des biomolécules marquées par des isotopes dans des applications thérapeutiques et diagnostiques, notamment en spectroscopie et, de façon générale, comme traceurs.

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