Method for improving fluorescence image contrast

Details Method for improving fluorescence image contrast Method for improving fluorescence image contrast

G - Physics

01

N

G01N 21/64 (2006.01) A61B 5/1455 (2006.01) A61B 6/00 (2006.01) G01N 21/84 (2006.01) G06T 5/20 (2006.01)

Patent

CA 2594237

There is provided an improved method for enhancing fluorescence images of an object, such as a biological tissue, by selectively eliminating or reducing unwanted fluorescence from fluorophores other than the fluorophore of interest. The method is based on the measurement of the lifetime of fluorophores while preserving information related to the fluorescence intensity of the fluorophore of interest.

L'invention concerne un procédé amélioré destiné à renforcer les images en fluorescence d'un objet, par exemple un tissu biologique, qui consiste à éliminer ou à réduire sélectivement la fluorescence non recherchée provenant de fluorophores autres que celui qui présente un intérêt. Le procédé s'appuie sur la mesure de la durée de vie des fluorophores tout en préservant les informations se rapportant à l'intensité de fluorescence du fluorophore d'intérêt.

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