Leakage detecting method for automatic pipetting apparatus

Details Leakage detecting method for automatic pipetting apparatus Leakage detecting method for automatic pipetting apparatus

G - Physics

01

M

G01M 3/26 (2006.01) G01M 3/28 (2006.01) G01N 35/10 (2006.01)

Patent

CA 2143674

In a method for detecting leakage in a pipetting apparatus, the inner pressure of the pump connected to the nozzle tip is measured to discriminate the presence of leakage objectively. In step 202, distilled water 90 is aspirated. When the inner pressure P1 of the pump after aspiration is roughly equal to the atmospheric pressure, a leakage is determined. Further, in step 203, after the inner pressure P1 of the pump has been measured after aspiration in step 202, the tip 36 is kept stopped for 30 sec, for instance without raising the tip, and the inner pressure P2 of the pump is measured again by the pressure sensor. When this inner pressure P2 is different from the inner pressure P1 previously measured, a determination that there is a leakage is made. Further, in step 204, aspirated distilled water 90 is dispensed into the same test tube 62. The inner pressure of the pump is kept monitored by the pressure sensor. In this case, when the pressure changes abruptly, a determination that there is a leakage is made. The leakage detection is completed at the time whenever the leakage is detected.

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