Process for recovering protease

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C12N 9/50 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1099653

ABSTRACT OF THE DISCLOSURE Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a pro- tective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound into the aqueous medium by adding a polar organic solvent which is miscible with water and separating an insoluble material from the resulting suspension by a solid-liquid separation to isolate the protease.

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