Heat-releasable reagents for in vitro chemical reactions

Details Heat-releasable reagents for in vitro chemical reactions Heat-releasable reagents for in vitro chemical reactions

C - Chemistry, Metallurgy

12

N

C12N 11/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2130108

Heat-releasable reagent compositions are disclosed comprising a dispensable body formed from a coating material (D) which entraps a reagent material (B). The entrapped reagent material does not react until the dispensable body is heated to a min- imal temperature. When heated to the predetermined temperature, the reagent is released into the surrounding medium and be- comes available for reaction with other substances in the medium. The heat-releasable reagent compositions are particularly use- ful in various types of nucleic acid hybridizations, polymerase chain reactions (PCR), reverse transcriptase reactions (RTR), nucleic acid sequencing and product generating reactions such as colorimetric, fluorometric and chemiluminescent enzyme lab- eled immunoassays.

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