Nucleic acid amplification with allele-specific suppression...

Details Nucleic acid amplification with allele-specific suppression... Nucleic acid amplification with allele-specific suppression...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2747068

The present invention is an improved method of selective amplification of certain variants of the target sequence, enhanced by allele-specific suppression of amplification of one or more of the other variants of the target sequence. The improve-ment is accomplished by providing an oligonucleotide, capable of hybridizing to the desired variant of the target sequence with the lesser affinity than to the undesired variants of the target sequence and optionally, by providing chemically modified primers and hot-start conditions.

La présente invention concerne un procédé amélioré d'amplification sélective de certains variants de la séquence cible, ladite amélioration correspondant à l'inhibition, spécifique des allèles, de l'amplification d'un ou de plusieurs des autres variants de la séquence cible. Cette amélioration est réalisée au moyen d'un oligonucléotide, capable de s'hybrider au variant recherché de la séquence cible avec une affinité moindre que celle qu'il présente pour les variants non souhaités de la séquence cible et, éventuellement, en faisant appel à des amorces chimiquement modifiées et à des conditions de démarrage à chaud.

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