C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C07K 16/40 (2006.01) C12N 5/12 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2281262
A monoclonal antibody by which medullasin that is a kind of serine proteases existing in granulocytes, production process thereof and an immunoassay of human medullasin using the antibody are disclosed. The monoclonal antibody specifically recognizes human medullasin. The process for producing the anti-human medullasin antibody comprises culturing hybridomas prepared by cell fusion between antibody-producing cells recovered from an animal immunized with human medullasin and myeloma cells, and recovering anti-human medullasin monoclonal antibody which specifically recognizes human medullasin from the culture in which the hybridomas are cultured. The immunoassay utilizes the anti-human medullasin antibody.
Un anticorps monoclonal reconnaissant la médullasine, qui est un type de sérine protéase présente dans les granulocytes, un procédé de fabrication de celui-ci et une épreuve immunologique décelant la médullasine humaine à l'aide de l'anticorps sont divulgués. L'anticorps monoclonal reconnaît spécifiquement la médullasine humaine. Le procédé de fabrication de l'anticorps anti-médullasine humaine comprend la culture d'hybridomes préparés par fusion de cellules productrices d'anticorps prélevées chez un animal immunisé avec de la médullasine humaine et de cellules myéloïdes, et la récolte d'un anticorps monoclonal anti-médullasine humaine qui reconnaît spécifiquement la médullasine humaine provenant de la culture dans laquelle les hybridomes ont été mis en culture. L'épreuve immunologique fait appel à l'anticorps anti-médullasine humaine.
Aoki Yosuke
Katsuragi Hisashi
Suzuki Hideaki
Takahashi Kiyoshi
Dainichiseika Color & Chemicals Mfg. Co. Ltd.
Smart & Biggar
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