Method for selecting transformed cells

Details Method for selecting transformed cells Method for selecting transformed cells

C - Chemistry, Metallurgy

12

N

C12N 15/65 (2006.01) A01H 1/04 (2006.01) A01H 3/04 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2265570

It has been a practice to use nptII as a selective marker gene (drug-resistant gene) and kanamycin, G418, etc. as a selective drug in selecting rice transformants. However, only a poor selection efficiency can be established thereby and it has been therefore required to develop a highly efficient rice transformation system with the use of a novel combination. By using paromomycin as a novel selective drug, rice can be transformed at an extremely high efficiency.

Il est habituel d'utiliser nptII comme gène marqueur sélectif (gène de résistance aux médicaments) et la kanamycine, le G418, etc., comme médicaments sélectifs pour sélectionner des transformants de riz. Mais la sélection obtenue par cette technique a un rendement faible, ce qui oblige à développer un système de transformation du riz très efficace faisant appel à une nouvelle combinaison. L'utilisation de la paromomycine comme nouveau médicament sélectif permet de transformer le riz avec un rendement très élevé.

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