Medium and culture of embryonic stem cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/02 (2006.01)

Patent

CA 2579643

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

Dans l'état de la technique, les procédés pour cultiver des cellules souches embryonnaires humaines, nécessitent l'utilisation soit de cellules nourricières de fibroblastes, soit d'un milieu qui a été exposé à des cellules nourricières de fibroblastes, pour maintenir les cellules souches dans un état non différencié. Il a été découvert que si des taux élevés de facteur de croissance de fibroblastes, d'acide gamma amino butyrique, d'acide pipécholique, de lithium et de facteur de croissance de transformation, sont ajoutés au milieu dans lequel les cellules couches sont cultivées, les cellules souches restent non différenciées indéfiniment par de multiples passages, même sans cellules nourricières et sans milieu conditionné.

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