G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 1/28 (2006.01) G01N 33/48 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2745431
A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.
La présente invention porte sur un procédé permettant d'inverser une réticulation induite par fixation dans des échantillons de tissu qui ont été conservés pour un examen histologique. Le procédé met en jeu le placement du tissu fixé dans un liquide dans des conditions de température et de pression élevées qui sont suffisantes pour inverser la réticulation induite par fixation, restaurer l'antigénicité de protéines et permettre une analyse moléculaire et protéomique améliorée de l'échantillon de tissu conservé. L'invention porte également sur des procédés de traitement de tissus pour un examen histologique dans des conditions de pression élevée qui améliorent la perfusion de réactifs liquides dans le tissu et réduisent les temps de traitement globaux.
Cunningham Robert E.
Fowler Carol B.
Mason Jeffrey T.
O'leary Timothy J.
American Registry Of Pathology
Smart & Biggar
The Government Of The United States Of America As Represented By
The United States Government As Represented By The Department Of
LandOfFree
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