Methods and devices for detecting bacteriophage

Details Methods and devices for detecting bacteriophage Methods and devices for detecting bacteriophage

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2286463

This invention relates to devices and methods to quantitate bacteriophage. The method employs the use of a precipitable dye and a contrast-coloring dye to improve the visualization of viral plaques on a lawn of bacteria. A preferred device employs a water-hydratable powder, a precipitable dye and a contrast- coloring dye and the device includes a spacer sufficiently thick so that the cover over the device does not contact the bacteria growing in the substrate of the device.

L'invention concerne des dispositifs et procédés permettant de quantifier les bactériophages. Le procédé consiste à utiliser un colorant précipitable et un colorant contrastant afin de rendre plus visibles les plaques virales sur un tapis de bactéries. Selon un mode de réalisation préféré, le dispositif utilise une poudre hydratable à l'eau, un colorant précipitable et un colorant constrastant. Le dispositif comprend en outre un élément de séparation suffisamment épais pour que la feuille recouvrant le dispositif n'entre pas en contact avec les bactéries en culture dans le substrat du dispositif.

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