Method for isolation of rna from formalin-fixed...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2394618

Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue.

L'invention concerne des procédés d'isolation rapide, fiable et simple d'ARN à partir d'échantillons tissulaires incorporés dans de la paraffine et fixés par du formol. Il est possible d'utiliser l'ARN purifié de cette manière pour surveiller les taux d'expression génique. L'échantillon tissulaire peut être un tissu provenant d'une tumeur ou d'une autre pathologie.

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