Assay for serum cholinesterase

Details Assay for serum cholinesterase Assay for serum cholinesterase

C - Chemistry, Metallurgy

07

C

C07C 219/14 (2006.01) C07C 229/54 (2006.01) C12M 1/40 (2006.01) C12Q 1/26 (2006.01) C12Q 1/44 (2006.01) C12Q 1/46 (2006.01)

Patent

CA 2095495

Cholinesterase activity is determined by means of the choline obtained by the action of cholinesterase on a compound of the formula: (see formula I) wherein R and R1 can be the same or different, and can be alkyl, such as methyl, ethyl, propyl, butyl, isopropyl, etc.; alkoxy, such as methoxy, ethoxy, propoxy, butoxy, etc.; amino (-NR2R3), wherein R2 and R3 can be the same or different, and can be hydrogen, methyl, ethyl, or propyl, wherein X- is a methyl or an anion, such as halogen (chloro, bromo, or iodo) sulfate, nitrate and acetate; The method of the invention ensures a conveniently low rate of cholinesterase catalyzed hydrolysis and a high degree of accuracy. Certain compounds of formula (I) defined above are novel compounds which are claimed per se.

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