Purification of fibrinogen

Details Purification of fibrinogen Purification of fibrinogen

C - Chemistry, Metallurgy

07

K

C07K 14/745 (2006.01) C07K 14/75 (2006.01)

Patent

CA 2318440

The present invention provides a method of obtaining a fibrinogen enriched preparation. The method includes the following steps: (i) adding an effective amount of a sulphated polysaccharide (SPS) to a fibrinogen containing solution with to form a fibrinogen containing precipitate; and (ii) extracting fibrinogen from the fibrinogen containing precipitate from step (i) with a solution containing at least 0.1 M, and preferably at least 0.2 M, salt to obtain a fibrinogen enriched preparation.

L'invention concerne un procédé relatif à l'élaboration d'une préparation de fibrinogène enrichie, qui comprend les étapes suivantes: (i) adjonction d'une quantité efficace de polysaccharide sulfaté à une solution de fibrinogène pour former un précipité à base de fibrinogène; et (ii) extraction du fibrinogène depuis ledit précipité au moyen d'une solution dont la concentration en sel est d'au moins 0,1 M et de préférence au moins 0,2 M, de manière à obtenir une préparation de fibrinogène enrichie.

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