C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) A01N 1/02 (2006.01) A61D 19/00 (2006.01) A61J 1/00 (2006.01) A61K 35/52 (2006.01) A61K 35/56 (2006.01)
Patent
CA 2322623
The invention provides a method for freeze-drying spermatozoa to obtain at least one reconstituted spermatozoon whose head (nucleus) is capable of fertilizing an oocyte to produce a live offspring. The motility of spermatozoa which have been freeze-dried and stored in a vacuum at room temperature is not restored when rehydrated. Their plasma membranes are disrupted and they are all "dead" in the conventional sense. However, when they are injected microsurgically into oocytes, their nuclei transform into male pronuclei and participate in normal embryonic development.
L'invention concerne un procédé de lyophilisation de spermatozoïdes, servant à obtenir au moins un spermatozoïde reconstitué dont la tête (noyau) peut fertiliser un ovocyte afin de produire une descendance vivante. La mobilité des spermatozoïdes qui ont été lyophilisés et conservés sous vide à température ambiante n'est pas restaurée lors de leur réhydratation. Les membranes plasmatiques des spermatozoïdes sont rompues et ils sont tous "morts" au sens habituel du mot. Cependant, lorsqu'on les injecte par microchirurgie dans des ovocytes, leurs noyaux se transforment en pronucléus mâles et participent au développement embryonnaire normal.
Wakayama Teruhiko
Yanagimachi Ryuzo
G. Ronald Bell & Associates
University Of Hawai'i
LandOfFree
Development of normal offspring from oocytes injected with... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Development of normal offspring from oocytes injected with..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Development of normal offspring from oocytes injected with... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2041002