Method of quantifying tumour cells in a body fluid and a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2237589

The disclosure relates to a method of quantifying tumour cells in a body fluid. A reaction is first carried out with the sample under investigation by which the RNA component of the telomerase is specifically amplified. The quantity of amplified nucleic acid is then quantitatively determined. Also disclosed are suitable test kits.

L'invention concerne un procédé pour quantifier des cellules tumorales dans un volume humoral. Selon ce procédé, on commence à mettre en oeuvre une réaction avec l'échantillon à examiner. Par cette réaction, la composante ARN de la télomérase est spécifiquement amplifiée. Ensuite, la quantité d'acide nucléique amplifiée est dénombrée. L'invention concerne également les nécessaires d'essai adéquats.

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