Method for expansion of epithelial stem cells

A - Human Necessities – 61 – L

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A61L 27/38 (2006.01) C12N 5/00 (2006.01) A61F 2/14 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2406282

Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself provide simple and effective means to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

La transplantation de cellules souches épithéliales, cultivées ex vivo sur une membrane amniotique traitée spécialement, permet la production, conjointement avec ladite membrane, d'un greffon chirurgical à cellules souches épithéliales développées. La méthode de production dudit greffon et le greffon lui-même constituent un moyen simple et efficace de reconstruire un tissu endommagé, dont, de préférence, le tissu cornéen. La source de cellules souche épithéliales peut être un explant très petit provenant d'une biopsie de tissu autologue et allogénique sain. La membrane amniotique est traitée, de manière que la matrice extracellulaire soit conservée mais que ses cellules soient tuées.

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