Method for producing dendritic cells

Details Method for producing dendritic cells Method for producing dendritic cells

C - Chemistry, Metallurgy

12

N

C12N 5/0784 (2010.01) C12N 5/0735 (2010.01) A61K 35/14 (2006.01) A61K 35/54 (2006.01) A61K 39/00 (2006.01) A61P 37/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2350210

Embryonic stem cell-derived dendritic cells and genetically modified immature dendritic cells which are capable of maturation together with methods for their production comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which bring about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture are described. The cells may be genetically modified.

L'invention concerne des cellules dentritiques dérivées de cellules souches embryonnaires, et des cellules dentritiques immatures génétiquement modifiées qui sont capables de maturation, ainsi que leurs méthodes de fabrication qui consistent à: fournir une population de cellules souches embryonnaires; cultiver les cellules souches embryonnaires en présence d'une cytokine ou d'une combinaison de cytokines permettant de différencier les cellules souches embryonnaires des cellules dentritiques; et récupérer les cellules dentritiques de la culture. Les cellules peuvent être génétiquement modifiées.

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