Method and materials for introducing dna into prevotella...

Details Method and materials for introducing dna into prevotella... Method and materials for introducing dna into prevotella...

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C07K 14/195 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2110924

2110924 9221761 PCTABS00017 A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola) and an engineered P. ruminicola comprising expressible foreign DNA.

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