Microfluidic devices and methods of using same

B - Operations – Transporting – 01 – L

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B01L 3/02 (2006.01) B01L 99/00 (2010.01) G01N 1/10 (2006.01)

Patent

CA 2521171

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

L'invention a trait à divers dispositifs microfluidiques à base d'élastomère, et à des procédés d'utilisation et de fabrication de tels dispositifs. Certains desdits dispositifs possèdent des réseaux de sites de réaction facilitant les analyses à haut rendement. Certains dispositifs contiennent également des sites de réaction situés à l'extrémité de canaux sans issue, au niveau desquels des réactifs ont été précédemment déposés lors de la fabrication. Les réactifs sont mis en suspension une fois que l'échantillon a été introduit dans le site de réaction. Les dispositifs peuvent être utilisés avec divers dispositifs de chauffage, et peuvent ainsi servir à diverses analyses nécessitant une régulation de la température, notamment à des applications de thermocyclage telles que des réactions d'amplification des acides nucléiques, le génotypage et des analyses d'expression génétique.

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