Apparatus and method for thermal cycling nucleic acid assays

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/40 (2006.01) C12M 1/36 (2006.01) C12Q 1/68 (2006.01) G05D 23/13 (2006.01)

Patent

CA 2178644

The method and apparatus for thermal cycling of nucleic acid assys includes a blended fluid stream produced from a plurality of constant velocity, constant volume, constant temperature fluid streams wherein to provide a variable temperature, constant velocity, constant volume fluid stream which is introduced into a sample chamber for heating and cooling samples contained therein. By diverting and altering the ratio of the constant temperature fluid streams relative to one another, the blended fluid stream is rapidly variable in temperature, providing for almost instantaneous temperature change within the environment defined by the sample chamber.

Le procédé et l'appareil décrits permettent d'effectuer le cyclage thermique de dosages d'acides nucléiques et utilisent un écoulement de fluides mélangés obtenu à partir de plusieurs écoulements de fluides dont la vitesse, la température et le volume sont constants, ceci produisant un écoulement de fluide à température variable, vitesse constante et volume constant qu'on introduit dans une chambre à échantillons pour chauffer et refroidir les échantillons qui s'y trouvent. En déviant les écoulemnts de fluides à température constante et en modifiant le rapport existant entre ces derniers, on peut rapidement faire varier la température de l'écoulement de fluides mélangés, ce qui a pour effet de modifier presque instantanément la température dans l'environnement défini par la chambre à échantillons.

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