Methods for diagnosing early lyme disease

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07K 14/20 (2006.01) C07K 16/12 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2177356

The invention relates to DNA encoding Borrelia burgdorferi sensu stricto outer surface protein C. Purified and recombinant forms of a 23 kDa protein from a Connecticut isolate of B. burgdorferi are described. The 23kDa protein, referred to as p23 or OspC, can be used for immunodiagnostic assays for detection of early lyme disease. The protein, amino acid coding for the protein and DNA sequences can be used to prevent Lyme disease, to diagnose/detect B.Burgdorferi in human or animal tissues or body fluids. Antibodies specific for the protein can also be generated.

L'invention concerne un ADN codant pour la protéine C de surface extérieure de <u>Borrelia burgdorferi</u> au sens strict. Des formes purifiées et recombinées d'une protéine de 23kDa provenant d'un isolat du Connecticut de <u>B. burgdorferi</u> sont décrites. Cette protéine de 23 kDa, appelée p23 ou OspC, peut s'utiliser pour des dosages immunodiagnostiques en vue de la détection précoce de la maladie de Lyme. La protéine, l'aminoacide codant pour celle-ci et les séquences d'ADN peuvent s'utiliser pour prévenir la maladie de Lyme, et pour diagnostiquer/détecter <u>B. burgdorferi</u> dans des tissus humains ou animaux ou des liquides organiques. Des anticorps spécifiques contre cette protéine peuvent être produits.

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