C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/08 (2006.01) C12N 15/53 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12N 15/81 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01)
Patent
CA 2331777
This invention relates to the improved expression of evolved polynucleotide and polypeptide sequences encoding for eukaryotic enzymes, particularly peroxidase enzymes, in conventional or facile expression systems. Various methods for directed evolution of polynucleotide sequences can be used to obtain the improved sequences. The improved characteristics of the polypeptides or proteins generated in this manner include improved folding, without formation of inclusion bodies, and retained functional activity. In a particular embodiment, the invention relates to improved expression of the horseradish peroxidase gene and horseradish peroxidase enzymes.
L'invention concerne l'expression améliorée de séquences polynucléotidiques et polypeptidiques développées, lesquelles codent pour des enzymes eucaryotes, notamment des peroxydases, dans des systèmes d'expression classiques ou faciles. Pour obtenir les séquences améliorées, on peut utiliser différentes méthodes pour le développement dirigé de séquences polynucléotidiques. Les caractéristiques améliorées des polypeptides ou protéines ainsi générées sont les suivantes: meilleur repliement, sans formation de corps d'inclusion, et activité fonctionnelle retenue. Dans un mode de réalisation particulier, l'invention concerne l'expression améliorée du gène de la peroxydase du raifort et des enzymes de la peroxydase du raifort.
Arnold Frances H.
Lin Zhanglin
California Institute Of Technology
Smart & Biggar
LandOfFree
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