Transgenic seedless fruit and methods

Details Transgenic seedless fruit and methods Transgenic seedless fruit and methods

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/14 (2006.01) C12N 9/10 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2289869

The present invention provides methods and DNA constructs for the genetic engineering of plant cells to produce plants which produce substantially seedless fruit in the absence of exogenous growth factors (auxins or cytokinins) and in the absence of pollination. The substantially seedless fruits produced by the methods described herein are about the size of wildtype seeded fruit (or somewhat larger) and these fruits are equal to or superior to the wildtype seeded fruit with respect to solid content and flavor. The seedless fruits of the present invention are produced in transgenic plants which contain and express auxin or cytokinin biosynthetic genes, e.g., tryptophan oxygenase or isopentenyl transferase coding sequences expressed under the regulatory control of sequences directing preferential or tissue specific expression of a downstream gene in the ovaries or developing fruit.

Cette invention a trait à des techniques et à des produits de recombinaison d'ADN aux fins de la transformation par génie génétique de cellules végétales en vue de la production de plants donnant un fruit sensiblement sans pépins et ce, en l'absence de facteurs de croissance exogènes (auxines ou cytokinines) et de toute pollinisation. Les fruits sensiblement sans pépins, produits grâce aux techniques de l'invention et dont la taille est approximativement celle du fruit sauvage à pépins, sont, du point de vue du contenu solide et de la saveur, égaux au fruit sauvage à pépins, sinon supérieurs. Ces fruits sans pépins sont produits à partir de plants transgéniques contenant ou exprimant des gènes biosynthétiques d'auxine ou de cytokinine, la tryptophane oxygénase ou l'isopentényle transférase notamment, codant des séquences exprimées sous l'action régulatrice de séquences dirigeant une expression spécifique tissulaire ou préférentielle d'un gène en aval dans les ovaires ou dans le fruit en développement.

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