B - Operations – Transporting – 01 – J
Patent
B - Operations, Transporting
01
J
B01J 19/00 (2006.01) C07B 61/00 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2362699
The invention relates to a method for the specific photolytic deprotection of nucleoside derivatives that are immobilized on a substrate, especially for use in the production of DNA chips. Said method is characterized in that a gel or viscous liquid layer is applied on the nucleoside derivatives that are immobilized on a substrate. Said gel or viscous liquid contains one or more polymer compounds and at least one representative from the group comprising water, water/C1-C4 alcohol mixtures and polar aprotic solvents. For initiating the deprotection, the nucleoside derivatives are irradiated. This method favors a rapid, clean and complete removal of the photolabile protective groups from the nucleoside derivatives, which results in the required purity of the synthesized nucleotide or oligonucleotide sequences.
L'invention concerne une technique qui permet de séparer de manière ciblée les groupes protecteurs de dérivés nucléosidiques immobilisés sur un matériau support, notamment pour la fabrication des puces à ADN. Selon ladite technique, on applique sur les dérivés nucléosidiques immobilisés sur le matériau support une couche de gel ou d'un fluide visqueux contenant un ou plusieurs composés polymères et au moins de l'eau, un mélange eau/alcool C¿1?-C¿4? et/ou un solvant aprotique polaire; puis on expose lesdits dérivés à la lumière de façon à séparer photolytiquement les groupes protecteurs. La technique permet de séparer les groupes protecteurs des dérivés nucléosidiques d'une manière rapide, propre et totale, et ainsi d'obtenir la pureté nécessaire des séquences nucléotidiques ou oligonucléotidiques synthétisées.
Giegrich Heinreich
Stengele Klaus-Peter
Blake Cassels & Graydon Llp
Chemogenix Gmbh
Nigu Chemie Gmbh
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