Method and apparatus for automatically testing the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/18 (2006.01) C12M 1/34 (2006.01) G01N 35/02 (2006.01)

Patent

CA 2230609

A method of automatically testing the sensitivity of a paraffinophilic microorganism to different antimicrobial agents and concentrations thereof includes a plurality of receptacles (2-22) each containing an amount of liquid medium, and antimicrobial agent to be tested and the microorganism to be tested. A paraffin containing slide (32-52) is placed in each receptacle (2- 22) after which incubation is effected. A light scatter sensor (62) is employed to automatically and sequentially monitor the extent of microorganism growth on the slides (32-52) and the growth of each slide is employed to determine which antimicrobial agent and which concentration is effective to resist growth of the paraffinophilic microorganism. The slides (32-52) are moved sequentially in operative relationship with the light scatter sensor (62) while resisting rotational movement of the slides. Positive and negative control slides are preferably employed as is a computer (64) in receiving and processing the results of the slide testing. Related apparatus is disclosed.

Procédé permettant de tester automatiquement la sensibilité d'un micro-organisme paraffinophile à différents agents antimicrobiens et différentes concentrations de tels agents. Le procédé nécessite plusieurs récipients (2-22) contenant chacun une certaine quantité de milieu liquide, l'agent antimicrobien à tester et le micro-organisme à tester. Une lamelle revêtue de paraffine (32-52) est placée dans chaque récipient (2-22) après quoi il est procédé à l'incubation. Un capteur de dispersion de lumière (62) est employé pour observer automatiquement et de manière séquentielle l'ampleur de la croissance du micro-organisme sur les lamelles (32-52) et; la croissance observée sur chacune de ces lamelles permet de déterminer quel agent antimicrobien et quelle concentration sont efficaces pour résister à la croissance du micro-organisme paraffinophile. Les lamelles (32-52) sont déplacées de manière séquentielle par rapport au capteur de dispersion de lumière (62) et s'opposent à un mouvement de rotation. On utilise de préférence des lamelles témoin positives et négatives ainsi qu'un calculateur (64) qui reçoit et traite les résultats des tests sur les lamelles. L'invention porte également sur l'installation appropriée.

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