Heparin functional affinity supports

C - Chemistry – Metallurgy – 08 – B

Patent

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Details

C08B 37/10 (2006.01) B01J 20/22 (2006.01) B01J 20/32 (2006.01) C07K 1/22 (2006.01) C07K 14/81 (2006.01) C08B 37/00 (2006.01)

Patent

CA 2168038

The disclosure describes hydrazide functional polymers obtained by reaction of azlactone functional polymers with hydrazine. The hydrazine functional polymers are useful to react with carbonyl functional materials such as heparin to provide heparin functional polymers which are useful for purification, preparation, and investigation of substances with biological activity. Methods of making and using the heparin functional polymers as significantly improved affinity chromatography supports such as beads for the purification of antithrombin III are described.

Cette invention concerne des polymères fonctionnels d'hydrazide que l'on obtient en faisant réagir des polymères fonctionnels d'azlactone avec de l'hydrazine. Les polymères fonctionnels d'hydrazide peuvent réagir avec des matériaux fonctionnels de carbonyle, comme l'héparine, pour donner des polymères fonctionnels d'héparine que l'on peut utiliser pour la purification, la préparation, la séparation et l'analyse de substances présentant une activité biologique. Des procédés de fabrication et d'utilisation des polymères fonctionnels d'héparine en tant que supports de chromatographie d'affinité fortement améliorés, comme les billes pour la purification de l'antithrombine III, sont décrits.

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