Optical microscopy with phototransformable optical labels

G - Physics – 01 – N

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G01N 21/63 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2609653

First activation radiation is provided to a sample that includes phototransformable optical labels ("PTOLs") to activate a first subset of the PTOLs in the sample. First excitation radiation is provided to the first subset of PTOLs in the sample to excite at least some of the activated PTOLs, and radiation emitted from activated and excited PTOLs within the first subset of PTOLs is detecting with imaging optics. The first activation radiation is controlled such that the mean volume per activated PTOLs in the first subset is greater than or approximately equal to a diffraction-limited resolution volume ("DLRV") of the imaging optics.

Selon l'invention, un premier rayonnement d'activation est appliqué à un échantillon qui comprend des étiquettes optiques phototransformables pour activer un sous-ensemble d'étiquettes optiques phototransformables dans l'échantillon. Un premier rayonnement d'excitation est appliqué au premier sous-ensemble d'étiquettes optiques phototransformables dans l'échantillon pour exciter au moins une partie des étiquettes optiques phototransformables activées, et le rayonnement émis par les étiquettes optiques phototransformables activées et excitées dans le premier sous-ensemble d'étiquettes optiques phototransformables est détecté au moyen d'optiques d'imagerie. Le premier rayonnement d'activation est régulé de sorte que le volume moyen par étiquette optique phototransformable activée dans le premier sous-ensemble est supérieur ou approximativement égal à un volume de résolution limitée par la diffraction des optiques d'imagerie.

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