Ral-4, useful in the production of small proteins and peptides

Details Ral-4, useful in the production of small proteins and peptides Ral-4, useful in the production of small proteins and peptides

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/60 (2006.01) C07K 14/65 (2006.01) C12N 1/21 (2006.01) C12N 15/71 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2070994

2070994 9111523 PCTABS00006 This invention presents a novel strain of E. coli, RAL-4, which is a tryptophan auxotroph and shows enhanced expression of proteins and peptides. In addition, this invention presents a method for achieving enhanced expression of proteins and peptides either by the use of RAL-4 or via generation of a host cell auxotrophy for an amino acid not resident in the heterologous protein to be expressed. In an embodiment of the invention, IGF-I and GRF are exprressed at levels above that found in wild type controls

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