Protocol for simulated natural biofilm formation

Details Protocol for simulated natural biofilm formation Protocol for simulated natural biofilm formation

C - Chemistry, Metallurgy

12

M

C12M 1/14 (2006.01)

Patent

CA 2321258

This invention provides a methodology for controlled biofilm formation in accordance with a monitored protocol. In a typical embodiment, a natural biofilm inoculum is used to form a simulated natural biofilm on retrievable slides in an annular reactor. The monitor protocol in the annular reactor subjects the bacterial consortia of the simulated natural biofilm to simulated environmental conditions. The invention simulated natural biofilm on a slide surface has utility for the testing of formulated product activity for inhibition or removal of the simulated natural biofilm, thereby providing a reliable indicator of the relative activity of the products under natural environmental conditions.

L'invention concerne un procédé servant à créer un film biologique en fonction d'un protocole contrôlé. Dans un mode de réalisation, on utilise un inoculum de film biologique naturel afin de créer un film biologique naturel simulé sur des microplaquettes récupérables dans un réacteur annulaire. Ce protocole contrôlé dans le réacteur annulaire soumet les groupements bactériens du film biologique naturel simulé à des conditions d'environnement simulées. Ce film biologique naturel simulé créé sur une surface de microplaquette est utile pour contrôler l'activité d'un produit formulé afin d'inhiber ou de supprimer ce film biologique naturel simulé, ce qui constitue un indicateur fiable de l'activité relative des produits dans des conditions naturelles d'environnement.

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