Synthetic lipid rafts and methods of use

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) A61K 9/127 (2006.01) A61K 38/16 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01) C12N 15/88 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2681795

Compositions and methods for delivering cargo to cells are provided. One aspect provides a synthetic vesicle containing caveolin 1 or a fragment thereof in an amount effective to form lipid rafts in the vesicle. The synthetic vesicles can be used to deliver polynucleotides, proteins, therapeutic agents, or a combination thereof to specific membrane-bound compartments of a cell. In certain aspects, the synthetic vesicles can deliver cargo to cellular organelles such as mitochondria.

La présente invention concerne des compositions et des procédés permettant de délivrer un chargement à des cellules. Un aspect de la présente invention concerne une vésicule de synthèse contenant de la cavéoline 1 ou un fragment de celle-ci en une quantité efficace pour former des radeaux lipidiques dans la vésicule. Les vésicules de synthèse peuvent être utilisées pour délivrer des polynucléotides, des protéines, des agents thérapeutiques, ou une combinaison de ceux-ci, à des compartiments membranaires spécifiques d'une cellule. Dans certains aspects de l'invention, les vésicules de synthèse peuvent délivrer un chargement à des organites cellulaires tels que les mitochondries.

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