Method for testing a cell sample

Details Method for testing a cell sample Method for testing a cell sample

G - Physics

01

N

G01N 33/487 (2006.01) G01N 15/00 (2006.01) G01N 15/02 (2006.01) G01N 15/06 (2006.01) G01N 15/12 (2006.01) G01N 33/49 (2006.01)

Patent

CA 2240573

In this invention, a cell parameter, for example cell volume, is determined by subjecting one or more aliquots of a sample cell suspension to one or more alterations of at least one parameter of the cell environment to identify a point at which the cells achieve a particular shape to obtain a sample specific shape compensation factor. Preferably, the environmental parameter change is a reduction in osmolality.

L'invention concerne la mesure d'un paramètre cellulaire, par exemple le volume cellulaire, que l'on obtient en soumettant une ou plusieurs aliquotes d'une suspension de cellules d'un échantillon à une ou plusieurs altérations d'au moins un paramètre de l'environnement des cellules étudiées, pour mettre en évidence un point où les cellules prennent une forme particulière afin d'obtenir un facteur de compensation de forme spécifique à l'échantillon. De préférence, le changement du paramètre de l'environnement est une réduction de l'osmolalité.

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