Perlecan transgenic animals and methods of identifying...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A01K 67/027 (2006.01) A61K 49/00 (2006.01) C07K 14/47 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2257304

The invention provides a transgenic non-human animal expressing a perlecan encoding transgene. Also provided is a double-transgenic non-human animal expressing a perlecan and an amyloid encoding transgene. A method of screening for a compound which alters the rate or extent of amyloid deposition is additionally provided. The method consists of: (a) constructing a perlecan transgenic animal; (b) administering an effective amount of a test compound to said perlecan transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition. Finally, the invention provides a method of screening for a compound which alters the rate or extent of amyloid deposition. The method consists of: (a) constructing a perlecan/amyloid double-transgenic animal; (b) administering an effective amount of a test compound to said perlecan/amyloid double- transgenic animal; and (c) determining whether said test compound alters the extent or rate of amyloid deposition.

Cette invention se rapporte à un animal non humain transgénique exprimant un transgène de codage de perlecane. Cette invention se rapporte également à un animal non humain transgénique double exprimant un transgène de codage de perlecane et d'amyloïde. Un procédé pour trier un composé qui modifie la vitesse ou l'extension du dépôt d'amyloïde est en outre présenté. Ce procédé consiste: (a) à construire un animal transgénique de perlecane; (b) à administer une quantité efficace d'un composé test à cet animal transgénique de perlecane; et (c) à déterminer si ce composé test modifie l'étendue ou la vitesse du dépôt d'amyloïde. Cette invention se rapport enfin à un procédé pour trier un composé qui modifie la vitesse ou l'extension du dépôt d'amyloïde. Ce procédé consiste: (a) à construire un animal transgénique double perlecane/amyloïde; (b) à administrer une quantité efficace d'un composé test à cet animal transgénique double perlecane/amyloïde; et (c) à déterminer si ce composé test modifie l'étendue ou la vitesse du dépôt d'amyloïde.

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