Mutated skeletal actin promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.2, 195/1.2

C12N 15/69 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2032626

ABSTRACT A mutated skeletal actin promoter which differs from the naturally occurring DNA sequence by nucleotide substi- tution has been found to increase gene expression. The mutation sites are specific to the cis-acting regions of the gene. Double mutations created a strong promoter. Trans- formed myogenic cells had ten times the transcriptional activity over the wild type. The mutated skeletal actin promoter can be used to overly express polypeptides, par- ticularly in myogenic cells.

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