Single-molecule pcr on microparticles in water-in-oil emulsions

Details Single-molecule pcr on microparticles in water-in-oil emulsions Single-molecule pcr on microparticles in water-in-oil emulsions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2656022

Modulation of the viscosity of the oil phase of a microemulsion used for amplification of DNA on a bead increases the homogeneity of product beads and the amount of amplified DNA per bead. Moreover the number of separate microemulsion populations that can be formed in parallel is increased using multi-well plates and mixer mill disruptor machines designed to lyse biological samples.

La présente invention concerne la modulation de la viscosité de la phase huileuse d'une micro-émulsion utilisée pour l'amplification d'ADN à la surface d'une bille, qui augmente l'homogénéité des billes produites et la quantité d'ADN amplifié par bille. De plus, le nombre de populations de micro-émulsions indépendantes qui peuvent être formées en parallèle est accru à l'aide de plaques à puits multiples et d'appareils désintégrateurs de broyeur mélangeur conçus pour la lyse d'échantillons biologiques.

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