High molecular weight pullulan and method for its production

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/14 (2006.01) C08B 37/00 (2006.01) C12N 15/04 (2006.01) C12P 19/10 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 2080658

A method for obtaining a substantially biologically pure culture strain of Aureobasidium pullulans from a wild type strain by enriching the collected strain for organisms which grow as fungal yeastlike cells, growing colonies from isolated yeastlike cells and selecting those yeastlike cells which exhibit reduced pigmentation. Biologically pure culture strains obtained by the invention as well as methods for producing pullulan having decreased pigmentation and/or increased molecular weight are disclosed.

Méthode permettant d'obtenir une souche cultivée d'Aureobasidium pullulans extrêmement pure sur le plan biologique à partir d'une souche de type sauvage, en enrichissant la souche prélevée pour des organismes qui croissent comme des cellules fongiques de type levure, en cultivant des colonies à partir des cellules de type levure isolées, et en choisissant les cellules de type levure qui présentent une pigmentation réduite. Sont décrites les souches cultivées biologiquement pures obtenues grâce à cette invention ainsi que les méthodes pour produire A. pullulans présentant une pigmentation moindre ou un poids moléculaire accru.

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